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Z-globulin, a protein component of the myelin, was isolated by ion exchange chromatography from the spinal cord of sheep. The protein (fractionated by SDS/PAGE as 19 kilodaltons) was purified by Triton fractionation and then isolated by preparative electrophoresis. The protein was found to contain no fibrinolytic activity as it was unable to inactivate the fibrinolytic enzyme, streptokinase, and it was not hydrolyzed by plasmin, plasminogen activator, or neutral protease. To test if the protein was susceptible to proteolysis by enzymes other than plasmin or plasminogen activator, it was incubated in vitro with purified trypsin, chymotrypsin, pronase, and papain, as well as with enzymes derived from extracts of Fusobacterium necrophorum, Streptomyces griseus, and Aspergillus oryzae, and with enzymes derived from extracts of the snails Lymnaea stagnalis and Helix aspersa. Trypsin, chymotrypsin, and F. necrophorum-derived enzymes caused almost complete breakdown of the protein; however, only papain had any effect on the isolated protein. Papain produced the most extensive breakdown, and it was possible to detect four polypeptides; two degraded faster than the third, one degraded more slowly than the second, and the fourth was present throughout the incubation.Q:
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Swiss financial regulators are considering requiring the banks to withdraw this year, noting that the U.S. economy is in «a deep recession,» while the euro zone is mired in a sovereign-debt crisis.
Already, «bank branches in the United States» are closed, and officials are concerned about «threats to bank secrecy.»
Swiss bankers are reportedly furiously arguing the idea of a U.S. withdrawal is a preposterous notion. The bankers insist the United States is the leading market for Swiss financial services. According to Deutsche Bank, U.S. financial industry is now responsible for roughly 10 percent of all Swiss bank revenues.
But Switzerland shouldn’t be holding its breath waiting for the Swiss banks to leave. The U.S. has been very clear about its intention to get rid of Swiss banking secrecy. As Financial Times’ Oliver Bullough recently noted:
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